Xinuo-Phytase Technical data

Ingredient: Xinuo-Phytase 5000FTU/gm

Technical Characteristics and Specifications

Enzyme Activity Units and Measurement Standards

The enzyme activity of Phytase 5000FTU/gm is defined according to international standards as the amount of enzyme required to liberate 1 micromole of inorganic phosphorus per minute from a 5.0 mmol/L sodium phytate solution at pH 5.5 and 37°C. This standardization ensures consistent measurement and quality control across different production batches and manufacturers.

Thermal Stability and Processing Resistance

The thermal stability of Phytase 5000FTU/gm represents a significant technological advancement in enzyme stabilization technology. Advanced thermostable formulations demonstrate remarkable resistance to high-temperature processing, with some products retaining 96-98% activity after exposure to 85°C for 3 minutes.

Activity Retention

Storage stability under controlled conditions demonstrates excellent long-term viability. Studies conducted at 25±2°C and 60±5% relative humidity show minimal activity loss over 12 months, with most products maintaining over 95% of original activity.

pH Stability and Optimal Reaction Conditions

Phytase 5000FTU/gm demonstrates optimal activity across a broad pH range, with most products showing peak performance between pH 4.5-5.5. The enzyme exhibits remarkable stability under acidic conditions, retaining significant activity even at pH levels as low as 2.0-3.0.

Production Process and Quality Standards

The production of Phytase 5000FTU/gm employs sophisticated fermentation technologies using genetically modified microorganisms, primarily strains of Aspergillus niger, Escherichia coli, or Pichia pastoris. The fermentation process typically involves submerged liquid culture under controlled conditions.

Production Process Flow

Strain Selection & Optimization Genetically modified microorganisms (Aspergillus niger, E. coli, Pichia pastoris) with optimized productivity. Mutant strains show 47% productivity improvement (6,181 to 9,523 IU/L per day).2

Fermentation 

Submerged liquid culture in controlled bioreactors (3-14L lab scale, >100,000L industrial scale). 72-168 hour fermentation time with precise control of temperature, pH, and dissolved oxygen.

Downstream Processing 

Clarification (centrifugation/filtration) → Ultrafiltration concentration → Formulation with stabilizers (glycerol for liquid, maltodextrin for powder).

Quality Control & Compliance

Good Manufacturing Practices (GMP) per US FDA 21 CFR Part 110. Comprehensive testing for enzyme activity, microbiological safety, and heavy metal content.

Application Benefits and Mechanisms of Action

Phosphorus Utilization Enhancement

The primary mechanism of action involves the enzymatic hydrolysis of phytate (myo-inositol hexakisphosphate), the primary storage form of phosphorus in plant-based feed ingredients. Through successive dephosphorylation reactions, the enzyme catalyzes the release of inorganic phosphate groups.

Phosphorus Digestibility Improvements

Nutrient Bioavailability Improvements

Phytate functions as a powerful chelating agent, forming stable complexes with proteins, amino acids, and divalent cations that significantly reduce their bioavailability. Phytase 5000FTU/gm disrupts these complexes through phytate degradation.

Environmental Impact and Sustainability

The environmental benefits of Phytase 5000FTU/gm are primarily manifested through reduced nutrient excretion and decreased environmental pollution. Comprehensive studies across multiple animal species consistently demonstrate phosphorus excretion reductions of 30-50%.

Carbon Footprint Reduction

Life cycle assessment studies indicate that phytase utilization can reduce carbon emissions by nearly 50% compared to traditional phosphorus supplementation approaches. This reduction is achieved through decreased phosphate mining, reduced transportation requirements, and lower energy consumption in feed production.

Economic Benefits and Cost-Effectiveness

The economic advantages of Phytase 5000FTU/gm implementation are multifaceted, encompassing direct feed cost savings, improved production performance, and reduced environmental compliance costs.

Species-Specific Applications and Clinical Data

Poultry Applications

Broiler Chickens

Broiler chicken applications represent one of the most extensively studied and economically significant uses of Phytase 5000FTU/gm. Comprehensive research demonstrates consistent improvements in growth performance, feed efficiency, and bone quality.

Laying Hens

Laying hen applications focus primarily on maintaining consistent egg production while reducing phosphorus excretion and feed costs. Research demonstrates that 500 FTU/kg phytase supplementation can allow reduction of non-phytate phosphorus content to 1.2 g/kg.

Swine Applications

Weaner and Grower Pigs

Swine applications demonstrate particular efficacy in nursery and grower phases, where phosphorus requirements are high relative to body weight and phytate content in common feed ingredients can limit mineral utilization. Research shows dose-dependent improvements in growth performance and bone mineralization.

Key Findings in Swine Applications

Increasing phytase levels from 500 to 3000 FTU/kg produces linear improvements in final body weight, average daily gain, and daily feed intake, with quadratic improvements in feed conversion ratio. Each 250 FTU/kg increase in phytase supplementation contributes to measurable performance enhancements, making higher doses particularly beneficial in challenging production environments or when maximum phosphorus utilization is desired.